In this type of chromatography, retention is based to the attraction among solute ions and charged internet sites certain to the stationary section.Note: Should you have an interest, There exists a whole segment about UV-obvious spectroscopy on the location. This explores the question in the absorption of UV and visual light by organic compounds
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While in the ionization chamber the remaining molecules—a combination on the mobile period parts and solutes—undergo ionization and fragmentation. The mass spectrometer’s mass analyzer separates the ions by their mass-to-demand ratio (m/z). A detector counts the ions and shows the mass spectrum.. HPLC separation of a combination of flavonoids
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